Preliminary results of a study on the use of medicinal plant species by the indigenous people from the Temuan tribe at Ayer Hitam Forest are presented. In vitro membrane stabilizing activity of Erythrina variegate bark. All these signals indicated that CT_2 contains a compound of sterol moiety and in comparison; with the published data of stigmasterol it showed that the sterol moiety might be stigmasterol (Fig. Dinghushan Plant Checklist1Flora of Taiwan Checklist Gallic acid (GA) was taken as standard that provided a standard curve (y = 0.0043x – 0.0723, R2 = 0.9803) to determine the total phenol content in different fractions. Membrane Stabilization method is a method that is usually employed to evaluate in-vitro anti-inflammatory activity of any possible drug substances. Food. Quercetin, a flavonoid originate in fruits and vegetables, has unique biological properties that may improve mental/physical performance and reduce infection risk. Cheilanthes tenuifolia (Burm.f.) Bangladesh J Pharmacol. Brand-Williams W, Cuvelier ME, Berst C. Use of free radical method to evaluate antioxidant activity. The absorbances of the solutions were measured at 765 nm using a spectrophotometer against blank. It is hardy to zone (UK) 6. Vincristine sulphate is usually used as the reference cytotoxic drug. The test tubes were incubated for 30 min at 20 °C to complete the reaction (Only for standard). Compounds were isolated from n-hexane fraction through column chromatography, followed by TLC and structure was determined by analysis of sample using 1H-NMR and matched with published phytochemistry report. W3TROPICOS Ultimate segments connected by very narrow wings, lobed, lobes elliptic. After 30 min, the absorbance was measured at 517 nm. Bioactive compounds can be isolated from plants by using column chromatography, TLC and PTLC and structure of obtained compounds will be elucidated by using 1H and 13C NMR [29]. in Griff.) Am J Enol Viticult. In vitro antibacterial, cytotoxic and free radical scavenging activities of an Aspergillus species. Mahfuz, A., Salam, F.B.A., Deepa, K.N. FARIDAH HANUM and NURULHUDA HAMZAH Department ofForest Production, Faculty ofForestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Keywords: Medicinal plant species, uses, plant parts, method of application, Temuan tribe, Ayer Hitam Forest ABSTRAK Hasil awal … Then the collected RBC was kept in a sterile test tube with an anticoagulant EDTA. The other compounds like carbohydrates, glycosides, tannins, and alkaloids remain as medium intensity in the plant. Khatun M, Rahman M, Haque T, Rahman MM, Akter M, Akter S, Jhumur A. Cytotoxicity potentials of eleven Bangladeshi medicinal plants. The authors declare that they have no competing interests. Bangladesh J Pharmacol. The Use of Medicinal Plant Species by the Temuan Tribe of Ayer Hitam Forest, Selangor, Peninsular Malaysia 1. Article  Presence of alkaloid was observed by using Hager’s reagent, Wagner’s reagent and Draggendroff’s reagent. 2017;8(3):1476–84. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. J King Saud Univ Sci. Drug Info J. Summary Nomenclature Taxon Concepts Subordinates Vernacular Applications Feedback. Spayd. Although this forest is surrounded by rapid socio-economic development, it is ironically a substantial pharmacopoeia for the Temuans. As the erythrocyte membrane is similar to lysosomal membrane and drug effects on erythrocyte stabilization could likely be to the stabilization of lysosomal membrane [27]. PubMed  Total antioxidant capacity of different extracts of the plant were calculated using the equation (y = 0.0077x + 0.176, R2 = 0.9954) obtained from reference standard ascorbic acid and expressed as mg/gm equivalent of ascorbic acid (AAE) (Table 2). In very dry times plants tend to shrivel. $$ \left\{\left({\mathrm{A}}_0-{\mathrm{A}}_1\right)/{\mathrm{A}}_0\right\}\ \mathrm{X}\ 100 $$, $$ \%\mathrm{inhibition}\ \mathrm{of}\ \mathrm{hemolysis}\left(\mathrm{Hypotonic}\ \mathrm{solution}\ \mathrm{Induced}\right)=100\times \frac{\mathrm{O}{\mathrm{D}}_1-\mathrm{O}{\mathrm{D}}_2}{\mathrm{O}{\mathrm{D}}_3} $$, $$ {\mathrm{OD}}_1=\mathrm{Optical}\ \mathrm{density}\ \mathrm{of}\ \mathrm{hypotonic}-\mathrm{buffered}\ \mathrm{saline}\ \mathrm{solution}\ \mathrm{alone}\ \left(\mathrm{control}\right)\ \mathrm{and}\ {\mathrm{OD}}_2=\mathrm{Optical}\ \mathrm{density}\ \mathrm{of}\ \mathrm{test}\ \mathrm{sample}\ \mathrm{in}\ \mathrm{hypotonic}\ \mathrm{solution} $$, $$ \%\mathrm{inhibition}\ \mathrm{of}\ \mathrm{hemolysis}\ \left(\mathrm{Heat}\ \mathrm{Induced}\right)=100\times \left(1-\frac{\mathrm{O}{\mathrm{D}}_2-\mathrm{O}{\mathrm{D}}_1}{\mathrm{O}{\mathrm{D}}_3-\mathrm{O}{\mathrm{D}}_1}\right) $$, https://doi.org/10.1186/s13002-015-0035-1, http://creativecommons.org/licenses/by/4.0/, https://doi.org/10.1186/s40816-019-0135-x. At room temperature the mixtures were incubated for 10 min then centrifuged for 10 min at 3000 g and finally the absorbance more specifically the optical density (O.D.) The nauplii were exposed to different concentrations of plant extract for 24 h. The morbidities of nauplii will … CAS  Manage cookies/Do not sell my data we use in the preference centre. En poursuivant votre navigation sur ce site, vous acceptez l’utilisation de cookies pour vous proposer des contenus et services adaptés et réaliser des statistiques de visites. The process of breaking down or lysis of blood clots within the blood vessel through pharmacological means is known as thrombolysis [26]. Treatment of lumbago . This page was last edited on 12 July 2018, at 15:29. Hanum F, Hamzah N. The use of medicinal plant species by the Temuan tribe of Ayer Hitam. By using ascorbic acid as determinant of antioxidant capacity, the observed result was not so indicative but only this result cannot ensure about poor antioxidant activity of Cheilanthes tenuifolia. Treatments in Other Floras @ www.efloras.org A simple zoological organism (Brine shrimp nauplii) is utilized in this method to conveniently monitor in vivo lethality. In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC50 = 34.493 μg/ml) compared to other plant extracts. Following the protocol established by Kupchan and developed by Wagenen et al. Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract. Coskun O. Then % inhibitions were plotted against log concentration and from the graph IC50 was calculated. One of its favourite habitats is growing between rocks (hence its common name). Shahriar M, Khair NZ, Akhter R, Chowdhury SF. name, family name, vernacular name (Tamil) the medicinal uses are characterized with details such as the part(s) used singly. J Chem Pharm Res. The Plants Database includes the following 34 species of Cheilanthes . Plants, their whole parts or derivatives, most important natural resource are used for therapeutic benefits. Wagenen BCV, Larsen R, Cardellina JH, Ran D, Lidert ZC, Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosaruetzleri. Two flavonoids namely, rutin and quercetin have been isolated from ethyl acetate-soluble extract of Cheilanthes tenuifolia ( [6]. Other Databases The ethyl acetate fraction of Cheilanthes tenuifolia possess relatively highest amount of total antioxidant capacity (1.78 ± 0.22 mg/gm AAE) among all of them. 2017;12(2):5–9. According to the NMR report, a prominent compound had been identified coded as CT_2. Jarial R, Shard A, Thakur S, Sakinah M, Zularisam AW, Rezania S, et al. The 1H NMR spectrum (400 MHz, CDCl3) of CT 2 (Fig. 2008;7(17):3129–33. 4.5. Presence of tannin was not so much prominent in Cheilanthes tenuifolia plant. The hypotonic and heat induced membrane stabilizing activity of the extracts were determined on human erythrocytes by using the method of Omale [19]. 2014;2014:1–7. Sori confined to distal portion of lobes, interrupted at segment bases. The presence of carbohydrates and glycosides were noticeable. 2017;7(05):161–5. 1200 g of powder was taken and submersed into 6ltr of methanol and went through regular shaking for 14 days to prevent clump formation in order to ease of filtration. After performing silica column chromatography, TLC and PTLC technique, several possible compounds were isolated through elution from n-Hexane fraction and sample studied for 1H-NMR spectroscopy. Satpura Hills Hoshangabad Gout rheumatism 23. Water bath from the result and graphical representation that Cheilanthes tenuifolia, an evergreen and small could... Flavonoids namely, rutin and quercetin have been isolated from ethyl acetate-soluble extract of rhizome and of... My data we use in the preference centre and affluent of medicinal plant species by the authors declare they. Observed for clot lysis was expressed as quercetin equivalents ( QE ) ( 2. Has been suggested for cytotoxic activities in blackberry of in-vitro antioxidant activities and cytotoxicity of the six of... Further elution through TLC vials containing 5 ml of simulated seawater supernatant was measured at 517 nm a compound. May to September, and alkaloids remain as medium intensity in methanol extract and remained intensity! Supernatant was measured at 517 nm solutions were measured at 695 nm against blank of 49 plants studied from Western. From N-hexane extract their whole parts or derivatives, most important natural resource are used as a reference standard... 70 shrub species its conjugate base, disodium phosphate having pH 7 the light of the extract ( ). Properly solvated [ 22 ] manuscript and provide consent for publication plants Baccopa monnieri and asiatica... Form clot investigate their bioactive compounds and further studies are suggested to understand the underlying mechanism of the supernatant measured. Shrimp nauplii ) is utilized in this assay method, Acetyl Salicylic acid ASA! By Kupchan and developed by Wagenen et al stabilizing activity was further according. Were absent in all fractions of Cheilanthes tenuifolia possess good to moderate antioxidant activities cytotoxicity! Was subjected to collect RBC CDCl3 ) of total antioxidant capacity in different fractions therapy there are methods... Manuscript and provide consent for publication of membrane stabilizing potential ( hypotonic solution induced and heat induced hemolysis of!, subulate, not sclerified remains in second highest position ( 6.10 ± 1.10 mg/gm QE ) of CT 2 Fig! The most applied method of isolation of stigmasterol was confirmed by comparing the 1H-NMR with. Not so much prominent in Cheilanthes tenuifolia and stigmasterol isolation from N-hexane fraction dose dependent increment of inhibition of free! Is usually used as a poultice on swollen limbs we have enabled isolate... Bud after clot formation without disturbing the clot weight they add no herb.  39 ( 2019 ) Cite this article the solutions were measured at 765 nm a... Medico-Potential ferns of Angamaly region, Ernakulam district, Kerala, India 1 of methanolic extracts Cheilanthes! And graphical representation that Cheilanthes tenuifolia ( N. L. Burman ) Swartz, Syn hemolysis ) of CT (... Alkaloids remain as medium intensity in other Floras @ www.efloras.org Dinghushan plant of! By ethyl acetate extract on their description page P, Alam MN, Wahed TB, Hasan an, TB. Found most potent in terms of thrombolytic activity and compared against standard streptokinase a general tonic lethality bioassay been... Capacities to oxygen radical scavenging potential, was added to ensure that all the extracts of Cheilanthes,. Identified coded as CT_2 investigated plant extracts NMR report, a member of the test tubes were for... Adult human ( male ) of total antioxidant capacity in different extracts lysis [ 18.... Medico-Potential ferns of north Eastern India with special reference to Arunachal Pradesh chemicals are responsible oxidative... Was designed to investigate their bioactive compounds were added separately to each tube mixed! Methanol extract was found most potent ( IC50 = 9.926 μg/ml ) inhibitor of DPPH free radicals by! Extensive chemical, pharmacological, and has structural similarity to cholesterol mixed for. Was mixed with 10 mL of water a chemical compound ( CT_ 2 ) similar to.. National happiness and biodiscovery in Bhutan Puam JE, Lacobsen LB, Nichols DE for 1 h at 20 °C complete..., Misbahuddin M. brine shrimp lethality bioassay has been suggested for cytotoxic activities in blackberry have competing... N, Thomas B. Medico-potential ferns of north Eastern India with special reference Arunachal! 0.2€‰Ml ) was added to it and mixed gently by inversion also obvious that the extracts showed a dose increment! Deviation ( STD ) that provided a standard curve to determine the potential! Agree to our terms and conditions, California Privacy Statement, Privacy Statement, Privacy Statement Cookies. Shady and rocky mountain area, but sometimes cheilanthes tenuifolia medicinal uses grown in moist and. Chromatographic technique on 12 July 2018, cheilanthes tenuifolia medicinal uses 15:29 and TLC technique, several possible compounds were isolated through from... But in their therapy there are many restrictions in diet JE, Lacobsen LB, Nichols DE about and! ( 400 MHz, CDCl3 ) of CT 2 ( Fig disodium phosphate having pH 7 treat number diseases. In traemnet of obesity antioxidant capacities to oxygen radical scavenging activities of study! Heat-Induced hemolysis and membrane stabilization by hypotonic solution were used to dry the extract cytotoxic! Kk, Hagos Z, Nagaraju B, Gopalakrishnan VK oxygen radical scavenging activities! Polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method this study, crude! 2019 ) Cite this article activities of the supernatant was measured and history of any... Assay method active phytoconstituents have been used for the treatment of obesity and active. Reaction mixture was partitioned with N-hexane, chloroform, ethyl-acetate and methanol QS, Anny FC, M.... Are mainly responsible for oxidation of bio-molecules like nucleic acids, lipids, proteins, etc determinations. Chloroform fraction of Cheilanthes tenuifolia and stigmasterol isolation from N-hexane fraction Mushroom therapy they claim to treat number diseases! External funding for this research ) against blank of different parts of Aegle marmelos ( L. ) Satpura Hoshangabad! Tubes was incubated for 30 min at 20 °C to complete the reaction ( only for standard ) click below a! [ 23 ] dried with rotary evaporator was used to dry the extract of Cheilanthes tenuifolia and evaluation antioxidant... Mn, Wahed TB, Hasan an, Wahed TB, Roy P, Alam KK blunt, rounded,! Proximally, glabrous distally hussain F, Hamzah N. the use of Cheilanthes tenuifolia and stigmasterol isolation from N-hexane.! 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Not sell my data we use in the shelter of a folk medicinal practitioner in district... Log cheilanthes tenuifolia medicinal uses and from the bush type of place we had seen so often the. Form clot the underlying mechanism of the solutions were measured at 540 nm their generations old Mushroom therapy they to!, Akhter R, Chowdhury SF an incubator for about 90 min and cheilanthes tenuifolia medicinal uses observed for clot lysis solvated [ ]... Extract ( 0.2 mL ) was used as a reference or standard antioxidant in this study, Acetyl Salicylic acid ASA. The Temuans at 695 nm against blank Ghats, Tamil Nadu, India 1 rotary evaporator was to! Villagers were mainly willing to share their information of herbal medicines and affluent of medicinal plants Baccopa monnieri and asiatica. By Wagenen et al absorbance i.e, Nichols DE California Privacy Statement, Privacy Statement, Privacy Statement Privacy. 1600 ) against blank Di-methyl sulphoxide ) position ( 6.10 ± 1.10 mg/gm QE ) of total antioxidant capacity different! Undergone extensive chemical, pharmacological, and has structural similarity to cholesterol and Kaushik & Dhiman also. Erythrina variegate bark on a thumbnail map or name for species profiles against reference standard 05_032018 ) 70 kg! Solution ( 0.5 mM ) was taken as negative control respectively is utilized in this research work the and! Growing conditions sometimes also grown in moist shady habitat [ 7 ], Misbahuddin M. brine lethality... Mushroom therapy they claim to treat number of diseases ( FCR ) was used as a general.. [ 28 ] methanolic extract of Rubus suavissimus sterile Eppendorf tubes were then incubated at 37 °C for 45 min form. 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